Simon's "Adenovirus-Mediated Transfer of the CFTR Gene to Lung of non-human primates: toxicity study" 1993

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	===Abstract===		===Abstract===
-	*Just put an asterisk as the first character to make a bullet point.	+	*Prepared a pre-clinical study of gene transfer into the lungs of baboons.
-	**Put two asterices to make a sub point.	+	**Recombinant adenovirus vectors containing expression cassettes for human cystic fibrosis transmembrane conductance regulator (CFTR) and Escherichia coli ~-galactosidase (lacZ) were instilled through a bronchoscope into limited regions of lung in 14 baboons
-	*Put three single quote marks around text you want to be '''bold''', perhaps for '''new terminology'''.	+	*Results of toxicity studies were found using clinical laboratory tests, chest radiographs, and necropsy tests were used
-	*Put two single quotes around ''things that should be italicized''.	+	**These were used to detect adverse effects
		+	*** The only adverse effect noted was a mononuclear cell inflammatory response within the alveolar compartment of animals receiving doses of virus that were required to induce detectable gene expression.
		+	**Minimal inflammation was seen and 107 and 108 pfu (plaque forming units) per ml (infectious units per volume) but perivascular lymphocytic and histocytic infiltrate was seen at 109 and 1010
		+	***Intensity of inflammation increased between 4 and 21 days
		+	**** At its greatest intensity, there was diffuse alveolar wall damage with intra-alveolar edema (the airways were relatively spared)
		+	**Chest radiographs revealed alveolar infiltrates, but only in regions of lung having the greatest intensity inflammation
		+	* Concluded that adenovirus-mediated gene transfer into the lungs of baboons is associated with development of alveolar inflammation at high doses of virus
	===Introduction===		===Introduction===

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Revision as of 00:17, 5 March 2010

Abstract

• Prepared a pre-clinical study of gene transfer into the lungs of baboons.
  • Recombinant adenovirus vectors containing expression cassettes for human cystic fibrosis transmembrane conductance regulator (CFTR) and Escherichia coli ~-galactosidase (lacZ) were instilled through a bronchoscope into limited regions of lung in 14 baboons
• Results of toxicity studies were found using clinical laboratory tests, chest radiographs, and necropsy tests were used
  • These were used to detect adverse effects
    • The only adverse effect noted was a mononuclear cell inflammatory response within the alveolar compartment of animals receiving doses of virus that were required to induce detectable gene expression.
  • Minimal inflammation was seen and 107 and 108 pfu (plaque forming units) per ml (infectious units per volume) but perivascular lymphocytic and histocytic infiltrate was seen at 109 and 1010
    • Intensity of inflammation increased between 4 and 21 days
      • At its greatest intensity, there was diffuse alveolar wall damage with intra-alveolar edema (the airways were relatively spared)
  • Chest radiographs revealed alveolar infiltrates, but only in regions of lung having the greatest intensity inflammation
• Concluded that adenovirus-mediated gene transfer into the lungs of baboons is associated with development of alveolar inflammation at high doses of virus

Introduction