Crystal's "Administration of an adenovirus containing the human CFTR cDNA to the respiratory tract of individuals with cystic fibrosis" 1994

From Biol557

Revision as of 18:36, 3 March 2010 (edit) 149.166.132.29 (Talk) (→Introduction) ← Older edit		Revision as of 18:39, 3 March 2010 (edit) (undo) 149.166.132.29 (Talk) (→Abstract) Newer edit →
Line 1:		Line 1:
	===Abstract===		===Abstract===
-	*Just put an asterisk as the first character to make a bullet point.	+	* They administered a recombinant adenovirus vector (AdCFTR) containing the normal human CFTR cDNA into the nasal and bronchial epithelium of 4 individuals with CF.
-	**Put two asterices to make a sub point.	+	* They found the vector expresses the CFTR cDNA in the respiratory epithelium in vivo.
-	*Put three single quote marks around text you want to be '''bold''', perhaps for '''new terminology'''.	+	* At 2x109 pfu there was no recombination, complementation, shedding of the vector, or rise in antibody titres. Although, there was a transient and systemic pulmonary syndrome observed (possibly mediated by IL-6)
-	*Put two single quotes around ''things that should be italicized''.	+	* They saw no long term effects
	===Introduction===		===Introduction===

---

Revision as of 18:39, 3 March 2010

Abstract

• They administered a recombinant adenovirus vector (AdCFTR) containing the normal human CFTR cDNA into the nasal and bronchial epithelium of 4 individuals with CF.
• They found the vector expresses the CFTR cDNA in the respiratory epithelium in vivo.
• At 2x109 pfu there was no recombination, complementation, shedding of the vector, or rise in antibody titres. Although, there was a transient and systemic pulmonary syndrome observed (possibly mediated by IL-6)
• They saw no long term effects

Introduction

• CF is a common lethal hereditary disorder caused by a mutation on CFTR on chromosome 7
• The disorder is characterized by airway and gastrointestinal disease, the lung manifestations dominate
• The pathogenesis is clearly linked to the lack of CFTR in the respiratory epithelia
• Symptoms in first decade:
  • Thick mucus, colonization with infectious bacteria, and chronic airway inflammation
• One approach to prevent respiratory manifestations of CFTR is gene therapy (talked about in abstract)
• Gene therapy must be carried out in vivo, cannot be done ex vitro