Editing Simon's "Adenovirus-Mediated Transfer of the CFTR Gene to Lung of non-human primates: toxicity study" 1993

===Abstract===
*Prepared a pre-clinical study of gene transfer into the lungs of baboons.
**Recombinant adenovirus vectors containing expression cassettes for human cystic fibrosis transmembrane conductance regulator (CFTR) and Escherichia coli ~-galactosidase (lacZ) were instilled through a bronchoscope into limited regions of lung in 14 baboons
*Results of toxicity studies were found using clinical laboratory tests, chest radiographs, and necropsy tests were used
**These were used to detect adverse effects
*** The only adverse effect noted was a mononuclear cell inflammatory response within the alveolar compartment of animals receiving doses of virus that were required to induce detectable gene expression.
**Minimal inflammation was seen and 107 and 108 pfu (plaque forming units) per ml (infectious units per volume) but perivascular lymphocytic and histocytic infiltrate was seen at 109 and 1010
***Intensity of inflammation increased between 4 and 21 days
**** At its greatest intensity, there was diffuse alveolar wall damage with intra-alveolar edema (the airways were relatively spared)
**Chest radiographs revealed alveolar infiltrates, but only in regions of lung having the greatest intensity inflammation
* Concluded that adenovirus-mediated gene transfer into the lungs of baboons is associated with development of alveolar inflammation at high doses of virus

===Introduction===
*Adenovirus-base vectors have certain properties that make them attractive vehicles for human gene therapy
**Ability to transfer genetic material efficiently into lung epithelial cells
***Led them to be chosen for the first trials for human gene therapy for cystic fibrosis
*Success of trial will be determined by the level and duration of transgene expression and safety
**Performed studies on baboons in order to prepare for human trial